The Commons Speaker has refused a government request to hold a “yes” or “no” vote on its Brexit deal.
John Bercow said a motion on the deal had been brought before MPs on Saturday, and it would be “repetitive and disorderly” to debate it again.
Saturday’s sitting saw MPs vote to withhold approval of Boris Johnson’s deal until it has been passed into law.
The government said it was disappointed, but would go ahead with introducing the necessary legislation.
The prime minister’s official spokesman added: “The Speaker has yet again denied us a chance to deliver on the will of British people.”
The UK is due to leave the EU in 10 days, and while Mr Johnson and fellow EU leaders have agreed a new deal to allow that to happen, it cannot come into force until it is approved by both the UK and European parliaments.
The government has presented the law which would implement the Brexit deal to the Commons, and it will begin its parliamentary journey on Tuesday.